Journal: ACS Omega

Article Title: SNAP-Tag-Based Antibody–Drug Conjugates Targeting Epidermal Growth Factor Receptor 1, Epidermal Growth Factor Receptor 2, Trophoblast Cell-Surface Antigen 2, and Tissue Factor for Ovarian Cancer Treatment

doi: 10.1021/acsomega.5c10377

Figure Lengend Snippet: Different expression in ovarian cancer cell lines. The expression levels of EGFR, Trop2, TF, and Her2 in ovarian cancer cell lines were determined by flow cytometry. The cells were treated with anti-EGFR (EGFR monoclonal antibody, H11), anti-Trop2 (Trop2 monoclonal antibody, MR54), anti-TF (CD142 antibody), and anti-Her2 (ErbB2 monoclonal antibody, 3B5) antibodies, respectively, followed by incubation with a secondary antibody (Goat anti-Mouse IgG Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647). The cells were permeabilized to determine the Her2 expression according to manufacturer’s instructions.

Article Snippet: Briefly, 4 × 10 5 cells were collected and washed twice with PBS and then stained with anti-EGFR (H11, 0.5 μg, Thermo Fisher Scientific), anti-Trop2 (MR54, 1 μg, Thermo Fisher Scientific), or anti-TF (CD142, 10 μL, Miltenyi Biotec) antibodies in 200 μL of PBS for 30 min on ice.

Techniques: Expressing, Flow Cytometry, Incubation